This collaborative project is to determine the genomic DNA sequence of Ralstonia solanacearum Race 3 Biovar 2, a USDA Select Agent. This Select Agent has has been introduced repeatedly into the U.S. but has not become established. Race 3 Biovar 2 is of particular concern because it is more cold tolerant than Race 1 strains, which are already present in the U.S., and because of unknown pathogenicity factors that may reside in the Race 3 Biovar 2 genome.
The project is organized under the direction of Dean Gabriel. Strain UW551 was chosen because it was introduced into the U.S. on latently infected geranium and its taxonomic status and pathogenicity were extensively characterized independently by Caitilyn Allen and John Elphinstone. Initial DNA sequencing was performed by SeqWright (Houston, TX) and initial annotation by Integrated Genomics (Chicago, IL). Bioinformatics tools, website design and additional DNA sequencing were performed by the ICBR Genomics Core, directed by William Farmerie